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Rockland Immunochemicals collagen 2
Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) <t>collagen</t> <t>2</t> + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.
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Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.

Journal: JBMR Plus

Article Title: β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice

doi: 10.1093/jbmrpl/ziag021

Figure Lengend Snippet: Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.

Article Snippet: Decalcified histological sections of the intact femora were used for immunohistochemical staining of Collagen 2 + (rabbit anti-mouse Col2, 1:100, #600-401-104-0.1, Rockland), Collagen 10 + (rabbit anti-mouse Col10, 1:200, #ABIN1077945, Antibodies online), Runx2 + (rabbit anti-mouse Runx2, 1:50, #8486, Cell Signaling Technology), Proliferating Cell Nuclear Antigen ( PCNA + ) (rabbit anti-mouse PCNA, 1:100, #MA5-32051, Thermo Fisher Scientific) and β 2 -AR + (rabbit anti-mouse β 2 -AR, 1:100, #bs-0947R, Bioss) chondrocytes in the growth plates.

Techniques: Immunohistochemical staining, Staining

Long bone growth of 12-wk-old female Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes, and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05.

Journal: JBMR Plus

Article Title: β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice

doi: 10.1093/jbmrpl/ziag021

Figure Lengend Snippet: Long bone growth of 12-wk-old female Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes, and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05.

Article Snippet: Decalcified histological sections of the intact femora were used for immunohistochemical staining of Collagen 2 + (rabbit anti-mouse Col2, 1:100, #600-401-104-0.1, Rockland), Collagen 10 + (rabbit anti-mouse Col10, 1:200, #ABIN1077945, Antibodies online), Runx2 + (rabbit anti-mouse Runx2, 1:50, #8486, Cell Signaling Technology), Proliferating Cell Nuclear Antigen ( PCNA + ) (rabbit anti-mouse PCNA, 1:100, #MA5-32051, Thermo Fisher Scientific) and β 2 -AR + (rabbit anti-mouse β 2 -AR, 1:100, #bs-0947R, Bioss) chondrocytes in the growth plates.

Techniques: Immunohistochemical staining, Staining